Molecular Immunology

Publications

Selected publications 1990-1997

   
  

   

Sitia R, Neuberger M, Alberini C, Bet P, Fra A, Valetti C, Williams G, Milstein C. Developmental regulation of IgM secretion: the role of the carboxy-terminal cysteine. Cell, 1990; 60:781-90. -abstract-

Rubartelli A, Cozzolino F, Talio M, Sitia R. A novel secretory pathway for interleukin-1 beta, a protein lacking a signal sequence. EMBO J., 1990; 9:1503-10.-abstract-

Klausner RD, Sitia R. Protein degradation in the endoplasmic reticulum. Cell, 1990; 62:611-4. Review.

Alberini CM, Bet P, Milstein C, Sitia R. Secretion of immunoglobulin M assembly intermediates in the presence of reducing agents. Nature. 1990; 347:485-7.-abstract-

Valetti C, Grossi CE, Milstein C, Sitia R. Russell bodies: a general response of secretory cells to synthesis of a mutant immunoglobulin which can neither exit from, nor be degraded in, the endoplasmic reticulum. J. Cell Biol., 1991; 115:983-94.-abstract-

Sitia R, Meldolesi J. Endoplasmic reticulum: a dynamic patchwork of specialized subregions. Mol. Biol. Cell, 1992;3:1067-72. Review. -abstract-

Rubartelli A, Bajetto A, Allavena G, Wollman E, Sitia R. Secretion of thioredoxin by normal and neoplastic cells through a leaderless secretory pathway. J Biol Chem., 1992; 267:24161-4.-abstract-

Fra AM, Fagioli C, Finazzi D, Sitia R, Alberini CM. Quality control of ER synthesized proteins: an exposed thiol group as a three-way switch mediating assembly, retention and degradation. EMBO J., 1993;12:4755-61.-abstract-

Rubartelli A, Bonifaci N, Sitia R. High rates of thioredoxin secretion correlate with growth arrest in hepatoma cells. Cancer Res., 1995; 55:675-80.-abstract-

Bonifaci N, Sitia R, Rubartelli A. Nuclear translocation of an exogenous fusion protein containing HIV Tat requires unfolding. AIDS, 1995; 9:995-1000.-abstract-

Montero M, Brini M, Marsault R, Alvarez J, Sitia R, Pozzan T, Rizzuto R. Monitoring dynamic changes in free Ca2+ concentration in the endoplasmic reticulum of intact cells. EMBO J., 1995; 14:5467-75.-abstract-

Cals MM, Guenzi S, Carelli S, Simmen T, Sparvoli A, Sitia R. IgM polymerization inhibits the Golgi-mediated processing of the mu-chain carboxy-terminal glycans. Mol Immunol., 1996; 33:15-24.-abstract-

Alessio M, De Monte L, Scirea A, Gruarin P, Tandon NN, Sitia R. Synthesis, processing, and intracellular transport of CD36 during monocytic differentiation. J. Biol. Chem., 1996; 271:1770-5.-abstract-

Reddy P, Sparvoli A, Fagioli C, Fassina G, Sitia R. Formation of reversible disulfide bonds with the protein matrix of the endoplasmic reticulum correlates with the retention of unassembled Ig light chains. EMBO J., 1996;15:2077-85.-abstract-

Isidoro C, Maggioni C, Demoz M, Pizzagalli A, Fra AM, Sitia R. Exposed thiols confer localization in the endoplasmic reticulum by retention rather than retrieval. J. Biol. Chem., 1996; 271:26138-42.-abstract-

Selected Publications 1997-2000

   

1997

Bornemann KD, Brewer JW, Perez E, Doerre S, Sitia R and Corley B. Secretion of Soluble Pre-B Cell Receptors by Pre-B Cells. J Immunol, 1997; 158: 2551-2557.-abstract-

Cabibbo A, Consalez GG, Sardella M, Sitia R and Rubartelli A. Changes in gene expression during the growth arrest of HepG2 hepatoma cells induced by reducing agents or TGFß1. Oncogene, 1997; 16: 2935-2943.-abstract-

Gruarin P, Sitia R, Alessio M. Formation of one or more intrachain disulfide bonds is required for the intracellular processing and transport of CD36. Biochem J, 1997; 328: 635-642.-abstract-

Morgavi P, Bonifaci N, Pagani M, Castigliolo S, Sitia R and Rubartelli A. The association of HIV-1 Tat with nuclei is regulated by Ca2+ ions and cytosolic factors. J Biol Chem, 1997; 272: 11256-11260.-abstract-

Carelli S, Ceriotti A and Sitia R. Stringent thiol-mediated retention in B lymphocytes and Xenopus oocytes correlates with inefficient IgM polymerization. Eur J.Immunol, 1997; 27, 1283-1292.-abstract-

        

1998

Alessio M, Gruarin P, Castagnoli C, Trombotto C, Stella M. Primary ex vivo culture of keratinocytes isolated from hypertrophic scars as a means for biochemical characterization of CD36. Int J Clin Lab Res, 1998; 28: 47-54.-abstract-

De Lalla, C., Fagioli, C., Serafini Cessi, F., Smilovich, D. and Sitia, R. (). Biogenesis and function of IgM: the role of the conserved µ-chain tailpiece glycans. Molec. Immunol., 1998, 35: 837-45.-abstract-

Maggioni C, Carelli S, Cabibbo A, Fagioli C, Fra AM and Sitia R. Assembly and secretion of antibodies during B-cell development. Bratisl Lek Listy, 1998; 99: 419-25.-abstract-

Rubartelli A, Poggi A, Sitia R and Zocchi MR. HIV-1 TAT: a polypeptide for all seasons. Immunology Today, 1998; 19: 543-5.-abstract-

Schwarzer E, Alessio M, Ulliers D, Arese P. Phagocytosis of malarial pigment hemozoin impairs expression of MHC Class II antigen, CD54 (ICAM-1) and CD11c (p150-95 integrin) in human monocytes. Inf Imm, 1998; 66: 1601-1606.-abstract-

Sardella M, Navone F, Rocchi M, Rubartelli A, Viggiano L, Vignali G, Consalez GG, Sitia R and Cabibbo A. KIF3C, a novel member of the kinesin superfamily: sequence, expression and mapping to human chromosome 2 at 2p23. Genomics, 1998; 47: 405-408.-abstract-

Smilovich D, Malagolini N, Fagioli C, De Lalla C, Sitia R and Serafini-Cessi F. (). Differential expression of Gal_1,3Gal epitope in polymeric and monomeric IgM secreted by mouse myeloma cells deficient in _2,6-sialyltransferase. Glycobiology, 1998; 8: 841-848.-abstract-

         

    
     

1999

Fra AM, Mastroianni N, Mancini R, Pasqualetto E and Sitia R. Human caveolin-1 and caveolin-2 are closely linked genes colocalizes with WI-5336 in a region 7q31 frequently deleted in tumors. Genomics, 1999; 56: 355-356.-abstract-

Consalez GG, Cabibbo A, Corradi A, Alli C, Sardella M, Sitia R and Fesce R. Computer assisted gene fishing: a new approach to PCR-based differential screening, alternative to differential display. Bioinformatics, 1999; 15: 93-105.-abstract-

         

   
  

2000

Fra AM, Pasqualetto E, Mancini M and Sitia R. Genomic organization and transcriptional analysis of the human genes coding for caveolin-1 and caveolin-2. Gene, 1999; 243: 75-83. -abstract-

Mancini R, Fagioli C, Fra AM, Maggioni C and Sitia R. Degradation of unassembled soluble Ig subunits by cytosolic proteasomes: evidence that retrotranslocation and degradation are coupled events. FASEB J, 1999; 14: 769-778. -abstract-

Benham AM, Cabibbo A, Fassio A, Sitia R, Bulleid NJ and Braakman I. The CXXCXXC motif determines the folding, structure and stability of human Ero1-lalpha. EMBO J, 2000; 19: 4493-4502. -abstract-

Cabibbo A, Pagani M, Fabbri M, Rocchi M, Farmery MR, Bulleid NJ and Sitia R. ERO1-L, a human protein that favours disulfide bond formation in the endoplasmic reticulum. J Biol Chem, 2000; 275: 4827-4833. -abstract-

Pagani M, Fabbri M, Benedetti C, Fassio A, Pilati S, Bulleid NJ, Cabibbo A, Sitia R.Endoplasmic reticulum oxidoreductin 1-lbeta (ERO1-Lbeta), a human gene induced in the course of the unfolded protein response. J Biol Chem. 2000;275:23685-92. -abstract-

Gruarin P, Del Monte L, Alessio M. CD36 folding revealed by conformational epitope expression is essential for cytoadherence of Plasmodium falciparum infected red blood cells. Paras Imm, 2000; 22: 349-360.-abstract-

Gruarin P, Thorne RF, Dorahy DJ, Burns GF, Sitia R, Alessio M. CD36 is a ditopic glycoprotein with the N-terminal domain implicated in intracellular transport. Bioph Bioch Res Comm, 2000; 275: 446-454.-abstract-

Gruarin P, Ulliers D, Thorne RF, Alessio M. Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Molec Cell Bioch (2000, in press).

Kopito, R. and Sitia, R. Aggresomes and Russell bodies: Symptoms of cellular indigestion? EMBO Reports, 2000; 1, 225-231.-abstract-